Journal
FOOD CHEMISTRY
Volume 315, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.126171
Keywords
White pepper; Carbon dots; Ratiometric nanosensor; Coenzyme A; Cellular imaging
Funding
- National Natural Science Foundation of China [31660181]
- Natural Science Foundation of Hunan Province, China [2018JJ1043]
- Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety [2018TP1003]
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A new-style white pepper derived dual-emission carbon dots (CDs) with a quantum yield of 10.4% was designed and facile constructed with one-pot solvothermal method. The green emission (520 nm) had an efficient and special turn-on fluorescence sensing of coenzyme A (CoA) with the aid of Cu2+, while red emission (668 nm) barely changed and worked as reference. In the concentration range (0-150 mu M), relative fluorescence intensity ratios (F-520/F-668) showed excellent linear correlation with concentrations of CoA, and detection limit was as low as 8.75 nm. Moreover, the strategy has been successfully applied for label-free detection of CoA in real pig liver samples with good recoveries (93.3-108.0%). Notably, the synthesized CDs had durable fluorescence, low cytotoxicity, and good biocompatibility for cellular imaging, which demonstrated wide and promising applicability for biosensing and bioimaging in the future.
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