Journal
FISH PHYSIOLOGY AND BIOCHEMISTRY
Volume 46, Issue 5, Pages 1743-1757Publisher
SPRINGER
DOI: 10.1007/s10695-020-00825-5
Keywords
Scatophagus argus; Gonadotropin-releasing hormone; 17 beta-Estradiol; Reproduction; Feedback regulation
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Funding
- SouthernMarine Science and Engineering Guangdong Laboratory (Zhanjiang) [ZJW-2019-06]
- National Key R&D Program of China [2018YFD0901203]
- Special key projects of Guangdong science and Technology Department's science and technology innovation strategy [2018B030311050]
- National Natural Science Foundation of China [41706174, 31702326]
- Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Guangdong Ocean University [KFKT2019ZD07]
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Gonadotropin-releasing hormone (GnRH) is a key neuropeptide of the reproductive system. However, little is known about the role of GnRH in the spotted scat (Scatophagus argus). Here, three GnRH subtypes (cGnRH-II, sGnRH, and sbGnRH) were identified in the spotted scat. cGnRH-II and sGnRH were only expressed in the brains and gonads of both male and female fish, exhibiting a tissue-specific expression pattern, while sbGnRH was expressed at different transcription levels in all examined tissues. During ovarian maturation, hypothalamus-associated sbGnRH was upregulated, while the expression of sGnRH was variable and cGnRH-II first increased and then decreased. In vivo experiments showed that sbGnRH significantly promoted the expression of fsh and lh genes in a dose-dependent manner and exhibited a desensitization effect on lh expression at high concentrations. For sGnRH and cGnRH-II, only high concentrations could induce fsh and lh expression. Furthermore, treatment with highly concentrated sbGnRH peptide also induced fsh and lh expression, whereas the sGnRH and cGnRH-II peptides only induced fsh expression in vitro. 17 beta-Estradiol (E-2) significantly inhibited the expression of sbGnRH mRNA in a dose-dependent manner and did not impact sGnRH and cGnRH-II mRNA levels in vivo or in vitro. The inhibitory effect of E-2 on sbGnRH expression was attenuated by the estrogen receptor (ER) broad-spectrum antagonist (fulvestrant) and the ER alpha-specific antagonist (methyl-piperidinopyrazole), respectively, implying that the feedback regulation on sbGnRH is mediated via ER alpha. This study provides a theoretical basis for the reproductive endocrinology of the spotted scat by studying GnRH.
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