Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 101, Issue -, Pages 51-57Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2020.03.047
Keywords
Glyphosate; Lymphocytes; MiR 203/PI3K/AKT; Apoptosis
Funding
- China Agriculture Research System [CARS-41-17]
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Glyphosate is a widely used pesticide worldwide. The problem surrounding gly-phosate is worth investigating, especially with its increased use, and an increasing number of studies have found that the toxic effect of glyphosate is objective. MiR-203 was seldom found in fish diseases or glyphosate researches. This article aims to explore the effect of miR-203 on carp lymphocytes during glyphosate exposure. Therefore, acridine orange/ ethidium bromide (AO/EB) and flow cytometry were carried out to evaluate apoptosis, and we also detected CYPs (CYP1A1, CYP1B1, CYP1C), cytokine secretion (IL-1 beta, IL-8, IL-10, IFN-gamma, TNF-alpha), inflammatory factors (NF-kappa B, cox-2), and the expression of miR-203 and the PI3K/AKT pathway by RT-PCR and Western blot analyses. Our results demonstrated that glyphosate exposure could induce lymphocyte apoptosis via regulation of miR203 targeting of PI3K/AKT, which was accompanied by CYPs activation, abnormal cytokine expression and an inflammatory response. These results show that glyphosate is not nontoxic to fish and provide new insights for the usage of glyphosate as an herbicide in the future.
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