Journal
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 78, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.etap.2020.103382
Keywords
Acute promyelocytic leukemia; NMR; Daunorubicin; Metabolomics; NB4 cells
Funding
- National Natural Science Foundation of China [81560032]
- Guangxi Natural Science Fund [2018 GXNSFAA050152]
- Weihan Academician's Outstanding Youth Training Fund of Harbin Medical University
- Harbin Medical University Cancer Hospital [JY2015-02, JCQN 2018-04]
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The aim of this study was to determine several key metabolites as potential biomarkers of daunorubicin (DNR) treatment of acute promyelocytic leukemia (APL) using APL blasts and NB4 cells. Samples which were obtained from 16 newly diagnosed APL patients and human APL NB4 cell lines were exposed to increasing concentrations of DNR (0 mu M, 0.1 mu M, 0.5 mu M and 1.0 mu M). Electron microscopy and Nuclear Magnetic Resonance (NMR) spectroscopy confirmed that there were clear differences between controls and DNR-treated groups, with the resultant models having excellent predictive and discriminative abilities. Four metabolites meeting the biomarker requirements were identified. KEGG analyses revealed that these biomarkers were associated with the metabolism of fat, choline, and glucose. These findings offered vital information about the effects of chemotherapies on the whole body biochemistry which might be important for monitoring apoptosis and injury to cells in order to reduce chemotherapies-induced side effects.
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