Two-step optimization process for grass hydrolysate application as biodiesel feedstock with novel quality characteristics

A major obstacle to biodiesel commercialization is supplying feedback which increases production costs. The potential of some oleaginous yeast for conversion of waste materials to biodiesel feedstock can overcome this problem. In this study, a potential oleaginous yeast strain was used for single-cell oil (SCO) production. Two sets of experiments were designed for the optimization process. According to the results obtained from the first experiment, lipid production and lipid content of this strain increased from 1.96 g/L and 22.6% to 3.85 g/L and 35.18% by optimization of grass hydrolysis, respectively. The results of the second experiment indicate an increase in SCO production and lipid content to 7.28 g/L and 56.39%, respectively. These results were obtained when HNO(3)was used for substrate pre-treatment. Lipid analysis by gas chromatography-mass spectrometry showed a suitable and high potential of fatty acid profile for biodiesel production, which was then confirmed by evaluating the physicochemical properties of the biodiesel obtained in compliance with the US and EU standards. Consumption of microbial oil and low-cost substrate can compensate the high costs of feedstock in biodiesel production.