Stimulations inducing tethered lipid bilayer formation on protein-modified substrate

Supported lipid bilayer (SLB) is one artificial biomembrane system used to study transportation reactions through biomembranes using solid devices and surface scientific methods. A key step in the SLB formation is the transformation of spherical lipid vesicles to a planar membrane. We investigated stimulation methods for adsorbed lipid vesicles on a streptavidin-modified SiO2/Si substrate to induce tethered-type SLB. We describe the effects of osmotic stress, Ca(2+)addition, and a freeze-thaw cycle on the adsorbed vesicles, and on the condition of t-SLB.