Journal
DEVELOPMENT
Volume 147, Issue 14, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.189134
Keywords
m(6)A; YTH domain; ECT2; ECT3; ECT4; Plant organogenesis
Categories
Funding
- European Research Council [ERC-2016-CoG 726417]
- Danmarks Frie Forskningsfond [FNU DFF2 9040-00409B]
- Novo Nordisk Fonden [NNF16OC0021712]
- BBSRC [BB/P020747/1, BB/M004112/1, BBS/E/J/000PR9787] Funding Source: UKRI
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mRNA methylation at the N6-position of adenosine (m(6)A) enables multiple layers of post-transcriptional gene control, often via RNA-binding proteins that use a YT521-B homology (YTH) domain for specific m(6)A recognition. In kabidopsis, normal leaf morphogenesis and rate of leaf formation require m(6)A and the YTH-domain proteins ECT2, ECT3 and ECT4. In this study, we show that ect2/ect3 and ect2/ect3/ect4 mutants also exhibit slow root and stem growth, slow flower formation, defective directionality of root growth, and aberrant flower and fruit morphology. In all cases, the m(6)A-binding site of ECT proteins is required for in vivo function. We also demonstrate that both m(6)A methyltransferase mutants and ect2/ect3/ect4 exhibit aberrant floral phyllotaxis. Consistent with the delayed organogenesis phenotypes, we observe particularly high expression of ECT2, ECT3 and ECT4 in rapidly dividing cells of organ primordia. Accordingly, ect2/ect3/ect4 mutants exhibit decreased rates of cell division in leaf and vascular primordia. Thus, the m(6)A-ECT2/ECT3/ECT4 axis is employed as a recurrent module to stimulate plant organogenesis, at least in part by enabling rapid cellular proliferation.
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