Journal
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
Volume 22, Issue 5, Pages 638-645Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1389201021666200703200126
Keywords
BCL2; MCL1; SNP; Chemotherapy; Colorectal cancer; Jordan
Funding
- Jordan University of Science and Technology, Irbid, Jordan [156/2013]
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The study investigated three SNPs in the promoter region of antiapoptotic genes in Jordanian patients with colorectal cancer and found that BCL2 rs2279115 and MCL1 rs9803935 SNPs were significantly distributed and associated with disease progression and metastasis, while BCL2 rs4987852 SNP showed no association with disease variables.
Background: Colorectal cancer is one of the most common types of cancer worldwide and a leading cause of death in Jordan. BCL-2 and MCL-1 are anti-apoptotic proteins that inhibit programmed cell death and their over-expression has been shown to be associated with reduced sensitivity to chemotherapy and poor survival in cancer patients. Objectives: In the present study, three SNPs in the promoter region of antiapoptotic genes were investigated in an effort to inspect the occurrences of SNPs (rs2279115, rs4987852) in the promoter region of BCL2 and SNP (rs9803935) in the promoter region of MCL1 in Jordanian patients with CRC, and investigate correlations between BCL2 and MCL1 SNPs and clinical outcomes. Methods: PCR-restriction fragment length polymorphism (RFLP)-based analysis was used for samples genotyping. Results: The BCL2 rs2279115 and MCL1 rs9803935 SNPs showed significant distribution where mutant and hetero genotypes are more prominent in CRC patients. Additionally, the rs2279115 genotypes and alleles were associated with stages of disease, its recurrence and metastasis. The MCL1 rs9803935 genotypes were associated disease metastasis. However, for BCL2 rs4987852 SNP, there was no association of genotypes or alleles with any of the disease variables. Conclusion: The BCL2 SNPs (rs2279115) and MCL1 SNP (rs9803935) present as important determinants of the progress of CRC in Jordanian patients.
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