Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 191, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2020.110985
Keywords
Titanium dioxide nanofibers; Circulating tumor cells; AS1411 aptamer; Nucleolin; Efficient capture
Funding
- National Natural Science Foundation of China [21575154, 21904135, 21775160]
- Science Foundation of Jiangsu Province [BK20180250, BE2018665]
- Science Foundation of Jiangxi Province [20192ACB21033]
- CAS International Cooperation Key program [121E32KYSB20170025]
- Jiangsu Province Six Talent Peaks program
- CAS/SAFEA International Innovation Teams program
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Herein, we developed an inexpensive titanium dioxide (TiO2) nanofiber substrate for efficient and selective capture of circulating tumor cells (CTCs) from mimic patients' samples. The TiO2 nanofiber substrates were fabricated by electrospinning in combination with the calcination process. The surface of nanofiber substrates was modified with the anti-adhesion molecule, bovine serum albumin (BSA) and the nucleolin aptamer AS1411, wherein, aptamer AS1411 specifically binds to the nucleolin protein overexpressed on the membrane surface of cancer cells. The formed TiO2 nanofiber substrates exhibited high efficacy and specificity to capture nucleolin positive cells through synergistic topographic interactions. Using the rare number of cell capture experiments, the capture efficiency of up to 75 % was achieved on the surface of the nanofiber substrate for rare number target cells spiked in the white blood cells (WBCs) from 1 mL whole blood samples. In conclusion, this study highlighted the potential of the TiO2-BSA-biotin-AS1411 nanofiber substrate as a highly efficient platform to realize the selective and specific capture of rare CTCs in the clinical settings.
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