Journal
CLINICAL & TRANSLATIONAL ONCOLOGY
Volume 23, Issue 2, Pages 257-264Publisher
SPRINGER INT PUBL AG
DOI: 10.1007/s12094-020-02413-8
Keywords
miR-22; Viability; EMT; PI3K; AKT; Ovarian cancer
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In this study, it was found that miR-22 was downregulated in ovarian cancer, and its impairment of cell proliferation and metastasis was attributed to its interaction with the NLRP3/PI3K/AKT signaling pathway, with potential prognostic implications. NLRP3 also played a regulatory role in this process.
Purpose miR-22 plays a great role in inhibiting cell growth, metastasis and enhanced cell apoptosis in several cancers. The purpose of this study was to investigate the functions of miR-22 in ovarian cancer. Methods The proliferative ability was measured using CCK-8 assay. The protein expression associated with EMT and PI3K/AKT signaling biomarkers were measured by western blot. Luciferase assay applied to measure the luciferase activity. Kaplan-Meier method was performed to evaluate the overall survival rate of ovarian cancers. Results miR-22 was low expressed and NLRP3 was overexpressed in ovarian cancer tissues and cells, and downregulation of miR-22 was associated with poor prognosis. The expression of NLRP3 had a negative correlation with miR-22 expression in ovarian cancer. miR-22 promoted cell viability and EMT through directly binding to the 3 '-UTR of NLRP3 mRNA and inhibited PI3K/AKT signaling pathway. NLRP3 partially restored functions of miR-22 on cell proliferation and EMT in ovarian cancer. Conclusion miR-22 impaired cell viability and EMT by NLRP3 and inhibited PI3K/AKT signaling pathway in ovarian cancer. The newly identified miR-22/NLRP3/PI3K/AKT axis provides novel insight into the pathogenesis of ovarian cancer.
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