Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 26, Issue 64, Pages 14556-14559Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.202002787
Keywords
biomimicry; molecularly imprinted polymer; nanoparticles; photo-polymerization; protein imprinting
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Funding
- Centro Piattaforme Tecnologiche (CPT) of the University of Verona
- Institut Universitaire de France
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Molecularly imprinted polymer nanoparticles (MIP NPs) are antibody-like recognition materials prepared by a template-assisted synthesis. MIP NPs able to target biomolecules, like proteins, are under the spotlight for their great potential in medicine, but efficiently imprinting biological templates is still very challenging. Here we propose generating a molecular imprint in single NPs, by photochemically initiating the polymerization from individual protein templates. In this way, each protein molecule tailors itself its own polymeric dress. For this, the template protein is covalently coupled with a photoinitiator, Eosin Y. Irradiated with light at 533 nm, the Eosin moiety acts as an antenna and transfers energy to a co-initiator (an amine), which generates a radical and initiates polymerization. As a result, a polymer network is forming only around the very template molecule, producing cross-linked NPs of 50 nm, with single binding sites showing high affinity (K(D)10(-9) m) for their biological target, and selectivity over other proteins.
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