Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 26, Issue 64, Pages 14536-14545Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.202000213
Keywords
antibiotics; antigens; aptamers; biological activity; biosensors
Categories
Funding
- Ministry of Science, Research and Arts of the state of Baden-Wurttemberg
- Baden-Wurttemberg Stiftung
- Federal Ministry of Education and Research
- European Union [CRC1279, CRC1074]
- Projekt DEAL
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Textbook procedures require the use of individual aptamers enriched in SELEX libraries which are subsequently chemically synthesized after their biochemical characterization. Here we show that this reduction of the available sequence space of large libraries and thus the diversity of binding molecules reduces the labelling efficiency and fidelity of selected single aptamers towards different strains of the human pathogenPseudomonas aeruginosacompared to a polyclonal aptamer library enriched by a whole-cell-SELEX involving fluorescent aptamers. The library outperformed single aptamers in reliable and specific targeting of different clinically relevant strains, allowed to inhibit virulence associated cellular functions and identification of bound cell surface targets by aptamer based affinity purification and mass spectrometry. The stunning ease of this FluCell-SELEX and the convincing performance of theP. aeruginosaspecific library may pave the way towards generally new and efficient diagnostic techniques based on polyclonal aptamer libraries not only in clinical microbiology.
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