Journal
CHEMISTRY AND PHYSICS OF LIPIDS
Volume 231, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.chemphyslip.2020.104945
Keywords
Nanodisc; Small-angle x-ray scattering; Bilayer interdigitation
Categories
Funding
- Graduate Assistantship in Areas of National Needs, U.S. Department of Education [P200A180065]
- NSF-CBET [1605971]
- National Institute of Health, National Institute of General Medical Sciences [P41 GM111244]
- Department of Energy Office of Biological and Environmental Research [KP1605010]
- NIH [S10 OD012331]
- DOE Office of Science [DE-SC0012704]
- NSF [DMR-0520547]
- European Union's Horizon 2020 research and innovation programme under the SINE2020 project [654000]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1605971] Funding Source: National Science Foundation
Ask authors/readers for more resources
The internal profile across the bilayer reveals important structural information regarding the crystallinity of acyl chains or the positions of encapsulated species. Here, we demonstrate that a simple five-layer-core-shell discoidal model can be employed to best fit the extended-q small angle X-ray scattering (SAXS) data and resolve the bilayer internal structure (with sub-nanometer resolution) of a nanoscale discoidal system comprised of a mixture of long- and short- chain lipids (known as bicelles). In contrast to the traditional core-shell discoidal model, the detailed structure in the hydrophobic core such as the methylene and methyl groups can be distinguished via this model. The refined model is validated by the SAXS data of bicelles whose electron scattering length density of the hydrophobic core is adjusted by the addition of a long-chain lipid with a fluorine-end group. The higher resolution of the bilayer internal structure can be employed to advance our understanding of the interaction and conformation of the membrane and associated molecules, such as membrane-associated proteins and locations of entrapped species in the lipid nanoparticles.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available