Journal
CHEMBIOCHEM
Volume 21, Issue 17, Pages 2540-2548Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202000341
Keywords
fast MAS; proteins; solid-state NMR spectroscopy
Funding
- Estonian Science Agency [PUT 1534]
- French Agence Nationale de Recherche [ANR-14-CE09-0024B]
- LABEX ECOFECT within the Universite de Lyon program Investissements d'Avenir [ANR-11-LABX-0048, ANR-11-IDEX-0007]
- Swiss National Science Foundation [200020_159707]
- European Research Council (ERC) under the European Union [741863]
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Spectral resolution is the key to unleashing the structural and dynamic information contained in NMR spectra. Fast magic-angle spinning (MAS) has recently revolutionized the spectroscopy of biomolecular solids. Herein, we report a further remarkable improvement in the resolution of the spectra of four fully protonated proteins and a small drug molecule by pushing the MAS rotation frequency higher (150 kHz) than the more routinely used 100 kHz. We observed a reduction in the average homogeneous linewidth by a factor of 1.5 and a decrease in the observed linewidth by a factor 1.25. We conclude that even faster MAS is highly attractive and increases mass sensitivity at a moderate price in overall sensitivity.
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