Journal
CEREBRAL CORTEX
Volume 30, Issue 11, Pages 5885-5898Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/cercor/bhaa164
Keywords
corpus callosum; fluorescent calcium; fMRI; interhemispheric inhibition; optogenetics
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Funding
- National Institutes of Health [1RF1NS113278, S10 RR023009]
- Max-PlanckSociety
- Deutsche Forschungsgemeinschaft [YU 215/3-1]
- Bundesministerium fur Bildung und Forschung [01GQ1702]
- National Institute of Neurological Disorders and Stroke
- China Scholarship Council
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [ZIANS002989] Funding Source: NIH RePORTER
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Optogenetically driven manipulation of circuit-specific activity enables causality studies, but its global brain-wide effect is rarely reported. Here, we applied simultaneous functional magnetic resonance imaging (fMRI) and calcium recording with optogenetic activation of the corpus callosum (CC) connecting barrel cortices (BC). Robust positive BOLD was detected in the ipsilateral BC due to antidromic activity, spreading to the ipsilateral motor cortex (MC), and posterior thalamus (PO). In the orthodromic target, positive BOLD was reliably evoked by 2 Hz light pulses, whereas 40 Hz light pulses led to reduced calcium, indicative of CC-mediated inhibition. This presumed optogenetic CC-mediated inhibition was further elucidated by pairing light pulses with whisker stimulation at varied interstimulus intervals. Whisker-induced positive BOLD and calcium signals were reduced at intervals of 50/100 ms. The calcium-amplitude-modulation-based correlation with whole-brain fMRI signal revealed that the inhibitory effects spread to contralateral BC, ipsilateral MC, and PO. This work raises the need for fMRI to elucidate the brain-wide network activation in response to optogenetic stimulation.
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