Synthesis of Mesoporous Siliceous Materials in Choline Chloride Deep Eutectic Solvents and the Application of These Materials to High-Performance Size Exclusion Chromatography

Mesoporous materials were prepared in choline chloride deep eutectic solvents by triblock copolymer-templated hydrothermal synthesis. The mesoporous materials were characterized by thermogravimetric analysis, scanning electron microscopy and nitrogen sorption analysis. The mesoporous material-based choline chloride-ethylene glycol (1:2 mol ratio) deep eutectic solvent had a flower-type shape, but the material-based choline chloride-urea (1:2) and based choline chloride-acetic acid (1:2) had mesoporous spherical shapes. These mesoporous materials were applied to high-performance size exclusion chromatography column packing. The separation effect of the two dextrans was better from the choline chloride-acetic acid deep eutectic solvent-based mesoporous spheres packed in a high-performance size exclusion chromatography column. In addition, the three polysaccharides, dextran-1 (molecular weight:670 KD), dextran-2 (molecular weight: 50 KD) and dextran-3 (molecular weight:5 KD), were separated successfully using the choline chloride-acetic acid deep eutectic solvent-based mesoporous spheres packed in a high-performance size exclusion chromatography column. The mesoporous spheres-based deep eutectic solvents are a potential packing material in high-performance size exclusion chromatography. The separation conditions of more target compounds will be explored in future studies.