Journal
CARBOHYDRATE POLYMERS
Volume 241, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.116269
Keywords
Glycerylphytate; Chitosan; Ionic crosslinking; Osteogenesis; Mesenchymal stem cells; Guided bone regeneration
Categories
Funding
- Ministry of Science, Innovation and Universities (Spain) [MAT2017-2017-84277-R]
- Instituto Salud Carlos III (ISCIII)-Fondo Europeo de Desarrollo Regional (FEDER)
- Centro de Investigacion Biomedica en Red en Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN)
- Fundacao para a Ciencia e a Tecnologia [UID/Multi/50016/2019, SFRH/BPD/116024/2016]
- Interreg V-A POCTEP Programme through FEDER European Union [0245_IBEROS_1_E.C.A.]
- Biotherapies: Bioengineered Therapies for Infectious Diseases and Tissue Regeneration [NORTE-01-0145-FEDER-000012]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/116024/2016] Funding Source: FCT
Ask authors/readers for more resources
Chitosan-based membranes are promising systems for guided bone regeneration. In this work, we used glycerylphytate as ionic crosslinker and osteinductor compound for the fabrication of chitosan membranes as supports for human mesenchymal stem cells. Three different glycerylphytate-crosslinked membranes were developed by changing the crosslinker concentration, from 2.5-10 wt-%, respect to chitosan. Physico-chemical characterization in terms of composition, morphology, and thermal behavior was further analyzed. Swelling degree, crosslinking density, and crosslinker release showed a glycerylphytate content-dependent behavior. Glycerylphytate suggested to improve osteointegration ability of chitosan surfaces by the formation of apatitelike aggregates after incubation in body simulated fluid. Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells. These results demonstrated a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available