Journal
CARBOHYDRATE POLYMERS
Volume 242, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.116394
Keywords
Marine green alga; Chaetomorpha linum; Sulfated arabinogalactan; Structural characterization; Anticoagulant activity; Coagulation; Factor
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Funding
- National Key Research and Development Program of China [2018YFC0310900]
- National Natural Science Foundation of China [41476108]
- Scientific and Technological Innovation Project by Pilot National Laboratory for Marine Science and Technology (Qingdao) [2016ASKJ08]
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A sulfated polysaccharide from the green alga Chaetomorpha linum , designated CLS4, was isolated by water extraction, anion-exchange and size-exclusion chromatography. Chemical and spectroscopic analyses demon-strated that CLS4 was a sulfated arabinogalactan, which was constituted by (1 -> 6)-beta-D-galactopyranose and (1 -> 5)-alpha-L-arabinofuranose residues with sulfate groups at C-2/C-3 of (1 -> 5)-alpha-L-arabinofuranose and C-2/C-4 of (1 -> 6)-beta-D-galactopyranose. CLS4 possessed strong anticoagulant activity in vitro or in vivo as evaluated by activated partial thromboplastin time and thrombin time assays. CLS4 largely inhibited the activities of the coagulation factors XII, XI, IX and VIII. CLS4 was a potent thrombin inhibitor mediated by antithrombin III (ATIII) or heparin cofactor II, and it also effectively stimulated the factor Xa inhibition by potentiating ATIII. Moreover, CLS4 had a high thrombolytic activity in vitro as assessed by clot lytic rate assay. The results suggested that CLS4 could be a promising source of anticoagulant agent.
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