Journal
BMC INFECTIOUS DISEASES
Volume 20, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12879-020-05202-4
Keywords
Campylobacter jejuni; T86I; gyrA; Ciprofloxacin; Antimicrobial resistance
Categories
Funding
- U.S. Department of Defense Global Emerging Infection Systems [WUN: 847705 82000 25GB B0016]
- Armed Forces Health Surveillance Center (AFHSC)
- Military Infectious Disease Research Program
Ask authors/readers for more resources
BackgroundCampylobacter jejuni is a leading cause of bacterial diarrhea worldwide, and increasing rates of fluoroquinolone (FQ) resistance in C. jejuni are a major public health concern. The rapid detection and tracking of FQ resistance are critical needs in developing countries, as these antimicrobials are widely used against C. jejuni infections. Detection of point mutations at T86I in the gyrA gene by real-time polymerase chain reaction (RT-PCR) is a rapid detection tool that may improve FQ resistance tracking.MethodsC. jejuni isolates obtained from children with diarrhea in Peru were tested by RT-PCR to detect point mutations at T86I in gyrA. Further confirmation was performed by sequencing of the gyrA gene.ResultsWe detected point mutations at T86I in the gyrA gene in 100% (141/141) of C. jejuni clinical isolates that were previously confirmed as ciprofloxacin-resistant by E-test. No mutations were detected at T86I in gyrA in any ciprofloxacin-sensitive isolates.ConclusionsDetection of T86I mutations in C. jejuni is a rapid, sensitive, and specific method to identify fluoroquinolone resistance in Peru. This detection approach could be broadly employed in epidemiologic surveillance, therefore reducing time and cost in regions with limited resources.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available