Journal
BLOOD
Volume 136, Issue 24, Pages 2764-2773Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood.2020006374
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Funding
- Leukemia and Lymphoma Society of Canada [417871]
- Terry Fox Foundation New Frontiers Program Project [1074]
- Canadian Cancer Society Research Institute [704257, 705047]
- Cancer Research Society [21339]
- Canadian Institutes of Health Research [CIHR EP1-120589, CEE-151619]
- Genome Canada under the Canadian Epigenetics, Environment and Health Research Consortium [C41EMT, C32EMT]
- Canadian Institutes of Health Research Frederick Banting and Charles Best Canada Doctoral Scholarships
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Hematopoietic clones with leukemogenic mutations arise in healthy people as they age, but progression to acute myeloid leukemia (AML) is rare. Recent evidence suggests that the microenvironment may play an important role in modulating human AML population dynamics. To investigate this concept further, we examined the combined and separate effects of an oncogene (c-MYC) and exposure to interleukin-3 (IL-3), granulocyte macrophage colony-stimulating factor (GM-CSF), and stem cell factor (SCF) on the experimental genesis of a human AML in xenografted immunodeficient mice. Initial experiments showed that normal human CD34(+) blood cells transduced with a lentiviral MYC vector and then transplanted into immunodeficient mice produced a hierarchically organized, rapidly fatal, and serially transplantable blast population, phenotypically and transcriptionally similar to human AML cells, but only in mice producing IL-3, GM-CSF, and SCF transgenically or in regular mice in which the cells were exposed to IL-3 or GMCSF delivered using a cotransduction strategy. In their absence, the MYC+ human cells produced a normal repertoire of lymphoid and myeloid progeny in transplanted mice for many months, but, on transfer to secondary mice producing the human cytokines, the many secondary producing cytokines, MYC+ cells rapidly generated AML. Indistinguishable diseases were also obtained efficiently from both primitive (CD34+CD382) and late granulocyte-macrophage progenitor (GMP) cells. These findings underscore the critical role that these cytokines can play in activating a malignant state in normally differentiating human hematopoietic cells in which MYC expression has been deregulated. They also introduce a robust experimental model of human leukemogenesis to further elucidate key mechanisms involved and test strategies to suppress them.
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