Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 84, Issue 11, Pages 2253-2263Publisher
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2020.1802221
Keywords
Alpha-lipoic acid; oxidative stress; inflammation; apoptosis
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Funding
- Key subjects of Hebei Province Health Department [ZL20140249]
- Science Foundation for Youths of the Higher Education Institutions of Hebei Province [QN2016137]
- Projects of Hebei Provincial Administration of Traditional Chinese Medicine [2016003]
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The study was aimed to investigate the effect of alpha-lipoic acid (ALA) on human umbilical vein endothelial cells (HUVECs) injury induced by hydrogen peroxide (H2O2) and to explore its possible mechanisms. We established the H2O2-induced HUVECs injury model and the ALA treatment groups in which HUVECs were co-incubated with H2O2(250 mu mol/L) and different final concentrations of ALA (100,200,400 mu mol/L) for 48 h. Cell survival rate assay and LDH activity assay were carried out. The levels of related proteins were performed by Western Blot. We observed that H(2)O(2)administration resulted in an increase in the LDH activity and a decrease in cell survival rate. The expression levels of Nox4, Bax, NF-kappa B p65, Caspase-9, Caspase-3, iNOS, VCAM-1 and ICAM-1 were up-regulated, while the expression level of Bcl-2 was down-regulated. All these factors were significantly improved by ALA treatment. In brief, ALA treatment ameliorates H2O2-induced HUVECs damage by inhibiting inflammation and oxidative stress.
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