Effects of citric acid on fermentation characteristics and bacterial diversity of Amomum villosum silage

To study the effects of citric acid on fermentation process of Amomum villosum silage, A. villosum was ensiled without or with 1%, 2% citric acid and fermentation parameters and bacterial diversity were analyzed after 3, 7, 14, 30 days ensiling, respectively. Citric acid treated silages had lower dry matter loss (1.83% vs 2.23%), pH (3.84 vs 6.02), ammonia-N (0.33 vs 1.79 g/kg DM) and coliform bacteria number (<2.00 vs 7.27 log10 CFU/g FM) than the control after 30 days ensiling. The relative abundance of lactic acid bacteria, Pediococcus and Lactobacillus increased, whereas undesirable microorganisms like Enterobacter, Escherichia-Shigella and Pantoea decreased in citric acid treated A. villosum silage. These results indicated that quality A. villosum silage could be obtained by citric acid addition.