Journal
BIORESOURCE TECHNOLOGY
Volume 308, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.123306
Keywords
Fed-batch culture; Fatty acid metabolism; Carbon conversion; Carbon availability; Astaxanthin
Funding
- Key Realm R&D Program of Guangdong Province [2018B020206001]
- Science and Technology Innovation Commission of Shenzhen [KQTD20180412181334790]
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To improve lipid and astaxanthin productivity without compromising biomass during the whole cultivation period, carbon-dependent kinetics involving nitrogen stress was applied under excess light to elevate intracellular carbon availability and metabolic activity of Chromochloris zofingiensis. Results suggested that fedbatch strategy proposed could increase lipid and astaxanthin productivity to 457.1 and 2.0 mg L-1 d(-1), respectively. Biomass productivity at 1084.3 mg L-1 d(-1) was comparable with that under suitable condition. Then C-13 tracer-based metabolic flux analysis (C-13-MFA) demonstrated that central carbon metabolism provided ATP, NADPH and carbon availability for lipid biosynthesis during the strategy. In combination with targeted metabolite analysis, C-13-MFA revealed that the strategy improved precursor content for lipid biosynthesis and elevated path rate to synthesize C16:0 and C18:0. The enhanced lipid content potentially accounted for the high biomass productivity. Therefore, comprehensively understanding relationships between carbon availability and carbon conversion could precisely design strategy for productivity improvements during cultivation.
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