Enhanced exopolysaccharide production in submerged fermentation ofGanoderma lucidumby Tween 80 supplementation

Bioactive polysaccharides extracted fromGanoderma lucidum(G. lucidum) have been widely applied in food and medicine for their multiple functions. In this study,G. lucidumexopolysaccharide (EPS) production in submerged fermentation was stimulated by Tween 80. The addition of 0.25% Tween 80 on day 3 gave a maximum production of mycelial biomass and EPS, with an increase of 19.76 and 137.50%, respectively. Analysis of fermentation kinetics showed that glucose was consumed faster after adding Tween 80, while the expression of EPS biosynthesis-related genes and ATP generation were greatly improved. Moreover, Tween 80 resulted in the significant accumulation of reactive oxygen species and increased cell membrane and cell wall permeability. The EPS from Tween 80-containing medium had higher contents of carbohydrate and uronic acid, lower molecular weight, and higher antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those of EPS produced in the absence of Tween 80. This study provides further evidence to clarify the stimulatory effects of Tween 80 in fermentation and provides a guide for the production of bioactiveG. lucidumEPS.

Automated summary

Addition of Tween 80 stimulated the production of bioactive polysaccharides from Ganoderma lucidum, resulting in higher levels of EPS and enhanced antioxidant activity in fermentation.