Journal
BIOPRESERVATION AND BIOBANKING
Volume 18, Issue 4, Pages 305-310Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/bio.2019.0105
Keywords
hypothermic storage; human umbilical vein endothelial cells; alginate; carboxymethyl chitosan; encapsulation
Funding
- National Key R&D Program of China [2018YFC0115500, 2018YFE0194500]
- National Natural Science Foundation of China [11627803]
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Human umbilical vein endothelial cells (HUVECs) have great potential in tissue engineering, regenerative medicine, and clinical applications. There is an ever-increasing demand to provide living HUVECs and HUVECs-hydrogel constructs to end users when needed in cell-based therapy and clinical applications. However, current methods to provide living cells and their constructs are mainly continuous culture and cryopreservation, which are high-cost, labor-intensive, time-consuming and inflexible. The research about hypothermic storage of HUVECs and their hydrogel constructs is still limited. Here, we studied the cell survival of HUVECs without encapsulation (W/O Encap) or with encapsulation (alginate, alginate with carboxymethyl chitosan [CMCH]) at 4 degrees C and 25 degrees C during 7 days, respectively. Also, we explored the optimal CMCH concentration for hypothermic storage, which is 0.5% (w/v) at 4 degrees C and 25 degrees C. Moreover, we evaluated the cell attachment after hypothermic storage. Our results enable the hypothermic storage of HUVECs and HUVEC-hydrogel constructs, and facilitate their application in tissue engineering and clinical medicine.
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