Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 34, Issue 10, Pages -Publisher
WILEY
DOI: 10.1002/bmc.4911
Keywords
biotransformation; hydrophilic; hydrophobic property; intestinal microbiome; metabolism signature; metabolites; Oplopanax horridus
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Funding
- National Institutes of Health/National Center for Complementary and Alternative Medicine (NIH/NCCAM) [AT004418, AT005362]
- Natural Science Foundation of Jiangsu Province [BK20160545]
- National Natural Science Foundation of China [81603378]
- 111 Project from the Ministry of Education of China
- State Administration of Foreign Experts Affairs of China [B16046]
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Oplopanax horridus, widely distributed in North America, is an herbal medicine traditionally used by Pacific indigenous peoples for various medical conditions. After oral ingestion, constituents inO. horridusextract (OhE) could be converted to their metabolites by the enteric microbiome before absorption. In this study, in order to mimic gut environment, the OhE was biotransformed using the enteric microbiome of healthy human subjects. For accurate and reliable data collection with optimized approaches in sample preparation and analytical conditions, ultra-performance liquid chromatography and quadrupole time-of-flight mass spectrometry were used to characterize parent constituents and their metabolites. In the extract, 20 parent compounds were identified including polyynes, sesquiterpenes, monoterpeondids, phenylpropanoids and phenolic acids. After the biotransformation, a total of 78 metabolites were identified, of which 37 belonged to polyynes metabolites. The common biotransformation pathways are hydroxylation, acetylization, methylation and demethylation. Based on the pathway distributions, the metabolism signature of OhE has been explored. The metabolism pathways of OhE compounds are dependent on their structural classifications and hydrophilic/hydrophobic properties. In summary, with comprehensive analysis, we systematically investigated human microbiome-derived OhE metabolites. The enteric microbial metabolism signature provides novel information for future effective use ofO. horridus.
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