Journal
BIOLOGICAL TRACE ELEMENT RESEARCH
Volume 199, Issue 5, Pages 1812-1822Publisher
HUMANA PRESS INC
DOI: 10.1007/s12011-020-02304-7
Keywords
Chaetomorpha linum; Silver nanoparticles; Colon cancer; HCT-116; Cytotoxicity; Apoptosis
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The study investigated the anticancer potential of silver nanoparticles synthesized from marine algae against colon cancer cells. The nanoparticles demonstrated dose-dependent cytotoxic effects on colorectal carcinoma cells and induced apoptosis through upregulation of pro-apoptotic proteins and downregulation of anti-apoptotic proteins. This biosynthesized silver nanoparticles were shown to be an efficient anticancer agent in inducing apoptosis in colon cancer cells.
The green approach of nanoparticle synthesis has gained more attention by researchers because of its nontoxic, eco-friendly, biocompatible, and sustainable nature. The present research investigated the anticancer effectiveness of silver nanoparticles synthesized from marine algaeChaetomorpha linum(C. linum) against colon cancer cell HCT-116 in vitro. Biosynthesized silver nanoparticles (C-AgNPs) are characterized using UV-spectrophotometry, dynamic light scattering (DLS), X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). We demonstrated the dose-dependent cytotoxic effect of C-AgNPs in human colorectal carcinoma cells (HCT-116) using MTT assay. The apoptosis induction in HCT-116 cells caused by C-AgNPs has studied fluorescence microscope by staining with fluorogenic agents 4 ',6-diamidino-2-phenylindole (DAPI), rhodamine 123, and 2 ',7 '-dichlorodihydrofluorescein diacetate (H(2)DCFDA). By using a flow cytometric test, the apoptotic action of C-AgNPs was performed. The immunoblotting study of caspases, as well as pro-apoptotic and anti-apoptotic protein expression, was studied using the PCR technique to understand the underlying molecular mechanism of C-AgNPs on cancer cells. The apoptotic studies showed an increase in the expression of apoptotic caspase 3, caspase 9, BH3-interacting domain death agonist (Bid), and Bax, along with a decrease in the anti-apoptotic protein like Bcl-2 and Bcl-xl, thereby veritably confirmed by immunoblotting and qPCR technique. The biosynthesized C-AgNPs was an efficient anticancer agent that can induce apoptosis in the HCT-116 colon cells.
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