Journal
BIOELECTROCHEMISTRY
Volume 133, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2020.107458
Keywords
Mycobacterium tuberculosis; ZnO nanoparticles; AuNPs; DNA biosensor; Label-free detection
Funding
- research council of Razi University
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In this study, a simple and cost-effective electrochemical DNA biosensor was developed for sensitive detection of mycobacterium tuberculosis (TB). Nanocomposite of zinc oxide (ZnO) and gold nanoparticles (AuNPs) was used as a platform for immobilizing thiolated TB DNA (probe DNA). ZnO was electrodeposited on a glassy carbon electrode by potentiostat electrolysis of Zn (NO3)(2) solution at -1.0 V (vs. Ag/AgCl), then AuNPs were loaded as the second layer at -0.4 V from HAuCl4 solution. Thiolated probe DNA was then covalently attached to AuNPs. Anodic peak current of Fe (CN)(6) (3 /4) was followed in hybridization experiments and a linear calibration curve was obtained in concentration range of 2.5-250 pM and limit of detection (LOD) of 1.8 pM for target DNA. The label-free TB biosensor exhibited high selectivity, suitable stability, and reproducibility. (C) 2020 Elsevier B.V. All rights reserved.
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