Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1861, Issue 7, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbabio.2020.148187
Keywords
Light harvesting; Excitation energy flow regulation; Single molecule spectroscopy; Stark spectroscopy; Photosynthesis
Categories
Funding
- European Research Council [267333]
- Foundation of Chemical Sciences part of NWO [700.58.305]
- National Equipment Programme of the National Research Foundation (NRF) [N00500, 87990]
- NRF Thuthuka programme [N00726, 94107]
- Photonics Initiative of South Africa
- Royal Netherlands Academy of Arts and Sciences (KNAW)
- Claude Leon Foundation
- Senior Postdoctoral fellowship at the University of Pretoria
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Phycobilisomes (PBs) absorb light and supply downstream photosynthetic processes with excitation energy in many cyanobacteria and algae. In response to a sudden increase in light intensity, excess excitation energy is photoprotectively dissipated in PBs by means of the orange carotenoid protein (OCP)-related mechanism or via a light-activated intrinsic decay channel. Recently, we have identified that both mechanisms are associated with far-red emission states. Here, we investigate the far-red states involved with the light-induced intrinsic mechanism by exploring the energy landscape and electro-optical properties of the pigments in PBs. While Stark spectroscopy showed that the far-red states in PBs exhibit a strong charge-transfer (CT) character at cryogenic temperatures, single molecule spectroscopy revealed that CT states should also be present at room temperature. Owing to the strong environmental sensitivity of CT states, the knowledge gained from this study may contribute to the design of a new generation of fluorescence markers.
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