Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 104, Issue 17, Pages 7239-7245Publisher
SPRINGER
DOI: 10.1007/s00253-020-10756-z
Keywords
Lignocellulose; Bioethanol; Ethyl acetate; Lipases; Alcohol acyl transferases
Categories
Funding
- National Key R&D Program of China [2018YFA0902200]
- National Natural Science Foundation of China [21978130, 31961133017, 21706125]
- Jiangsu Province Natural Science Foundation for Youths [BK2170993, BK20170997]
- Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture
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Ethyl acetate is one of the short-chain esters and widely used in the food, beverage, and solvent areas. The ethyl acetate production currently proceeds through unsustainable and energy intensive processes, which are based on natural gas and crude oil. Microbial conversion of biomass-derived sugars into ethyl acetate may provide a sustainable alternative. In this review, the perspectives of bio-catalyzing ethanol and acetic acid to ethyl acetate using lipases in vitro was introduced. Besides, the crucial elements for high yield of ethyl acetate in fermentation was expounded. Also, metabolic engineering in yeasts to product ethyl acetate in vivo using alcohol acyl transferases (AAT) was discussed.
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