Description of Erythrobacter mangrovi sp. nov., an aerobic bacterium from rhizosphere soil of mangrove plant (Kandelia candel)

A novel Gram-stain negative, aerobic, non-motile, rod-shaped bacterium, designated as strain EB310(T), was isolated from rhizosphere soil of mangrove plantKandelia candelin Fugong village, Zhangzhou, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain EB310(T)belonged to the genusErythrobacter, clustering withErythrobacter pelagiJCM 17468(T),Erythrobacter lutimarisKCTC 42109(T)andErythrobacter marisflaviKCTC 62896(T), and showed the highest 16S rRNA gene sequence similarity of 97.5% toErythrobacter pelagiJCM 17468(T). The genomic average nucleotide identity and in silico DNA-DNA hybridization values between strain EB310(T)and the reference strains were 71.0-75.5% and 19.8-20.0%, respectively. Growth ranges of the isolate occurred at 10-45 degrees C (optimum 28-30 degrees C), pH 5.5-9.5 (optimum pH 7.5) and 0-9.0% NaCl concentrations (optimum 2.0%, w/v). The strain did not produce bacteriochlorophyllaand flexirubin, but produced carotenoids. The strain contained Q-10 as the predominant ubiquinone and summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c) and summed feature 8 (C-18:1 omega 6c/C-18:1 omega 7c) as the major fatty acids. The major polar lipids were sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylcholine. Differential phenotypic characteristics, together with chemotaxonomic, phylogenetic and genomic distinctiveness, indicated that strain EB310(T)is distinguishable from other members of the genusErythrobacter. On the basis of the data exhibited, strain EB310(T)is considered to represent a novel species of the genusErythrobacter, for which the nameErythrobacter mangrovisp. nov., is proposed. The type strain is EB310(T)(= KCTC 72109(T) = MCCC 1K03690(T)). The genomic DNA G + C content is 62.9 mol%.