Journal
CHINESE JOURNAL OF PHYSIOLOGY
Volume 59, Issue 4, Pages 218-224Publisher
CHINESE PHYSIOLOGICAL SOC
DOI: 10.4077/CJP.2016.BAE401
Keywords
acetylcholine; botulinum toxin type A; pyloric smooth muscle; synaptosomal-associated protein of 25 kDa; toosendanin
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Funding
- Medical Subject Fund of Jiangnan University [JUSRP11572]
- Scientific and Technique Support Project of Gansu Province, PRC [090NKCA112]
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Botulinum toxin type A (BTX-A) selectively cleaves synaptosomal-associated protein of 25 kDa (SNAP-25) and results in inhibition of the fusion of synaptic vesicles containing neurotransmitters with the presynaptic membrane to undergo exocytosis and release. The aim of this study was to investigate whether BTX-A inhibited the pyloric smooth muscle contractility induced by acetylcholine (ACh) after BTX-A-mediated cleavage of SNAP-25 antagonized by toosendanin (TSN). Three groups of rat pyloric muscle strips were studied in vitro. All strips were allowed to equilibrate for 52 min under a basal loading tension of 1 g in Krebs solution and spontaneous contractile waves were recorded as their own controls before adding each drug. According to experimental protocols, 100 mu M ACh, 1 mu M atropine, 29.6 mu M TSN and 10 U/ml BTX-A was added, respectively. BTX-A directly inhibited pyloric spontaneous contraction and ACh-induced contractile response. Addition of 10 U/ml BTX-A still inhibited pyloric smooth muscle contractility following incubation of TSN, while subsequent administration of 100 mu M ACh had no effect. BTX-A inhibits pyloric smooth muscle contractility in our study suggesting BTX-A inhibits not only ACh release from cholinergic nerves but also muscarinic cholinergic muscular transmission.
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