Journal
ANTICANCER RESEARCH
Volume 40, Issue 6, Pages 3239-3246Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.14305
Keywords
NCAPH; condensin; chromosome; cell cycle; NSCLC
Categories
Funding
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning [NRF-2015R1A5A2009070]
Ask authors/readers for more resources
Background/Aim: Non-structural maintenance of chromosomes condensin I complex subunit H (NCAPH) is implicated in correct chromosome condensation and segregation during mitosis. However, the functional role of NCAPH in the pathogenesis of non-small-cell lung cancer (NSCLC) remains unclear. The aim of this study was to elucidate the role of NCAPH in NSCLC cells. Materials and Methods: A549 and H1299 NSCLC cells were transfected with small-interfering RNA (siRNA) against NCAPH. Subsequently, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony-formation assay and flow cytometry analysis were performed to reveal the role of NCAPH in NSCLC cells. In addition, migration and invasion assay were also performed. Results: NCAPH knockdown inhibited cell proliferation, induced cell-cycle arrest at G(2)/M phase, and prevented colony formation, migration and invasion by NSCLC cells. Conclusion: NCAPH is involved in NSCLC progression and development, and may be a potential therapeutic target for NSCLC treatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available