Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 42, Pages 18619-18626Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202005505
Keywords
DNA lattices; Holliday junctions; host-guest scaffolds; self-assembly; structural DNA nanotechnology
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Funding
- Howard Hughes Medical Institute
- Department of Energy Office of Science User Facility [DE-AC02-05CH11231]
- U.S. Department of Energy, Office of Biological and Environmental Research [DE-AC02-06CH11357]
- DOE Office of Science [DE-SC0012704]
- National Institute of Health, National Institute of General Medical Sciences (NIGMS) through a Biomedical Technology Research Resource P41 grant [P41GM111244]
- Arizona State University
- Air Force Office of Scientific Research [FA9550-17-1-0053]
- National Science Foundation Division of Materials Research [NSF2004250]
- DOE Office of Biological and Environmental Research [KP1605010]
- Presidential Strategic Initiative Fund from Arizona State University
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DNA is an ideal molecule for the construction of 3D crystals with tunable properties owing to its high programmability based on canonical Watson-Crick base pairing, with crystal assembly in all three dimensions facilitated by immobile Holliday junctions and sticky end cohesion. Despite the promise of these systems, only a handful of unique crystal scaffolds have been reported. Herein, we describe a new crystal system with a repeating sequence that mediates the assembly of a 3D scaffold via a series of Holliday junctions linked together with complementary sticky ends. By using an optimized junction sequence, we could determine a high-resolution (2.7 angstrom) structure containing R3 crystal symmetry, with a slight subsequent improvement (2.6 angstrom) using a modified sticky-end sequence. The immobile Holliday junction sequence allowed us to produce crystals that provided unprecedented atomic detail. In addition, we expanded the crystal cavities by 50 % by adding an additional helical turn between junctions, and we solved the structure to 4.5 angstrom resolution by molecular replacement.
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