Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 59, Issue 46, Pages 20651-20658Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202009387
Keywords
DNA hydrogels; DNA nanotechnology; i-motif DNA; organelle-like structure; self-assembly
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Funding
- National Natural Science Foundation of China [21622404, 21621004, 21905196, 31971305]
- National Key R&D Program of China [2019YFA09005800, 2018YFA0902300]
- Tianjin Natural Science Foundation (Basic research plan) [18JCJQJC47600, 19JCQNJC01900]
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The design of controllable dynamic systems is vital for the construction of organelle-like architectures in living cells, but has proven difficult due to the lack of control over defined topological transformation of self-assembled structures. Herein, we report a DNA based dynamic assembly system that achieves lysosomal acidic microenvironment specifically inducing topological transformation from nanoparticles to organelle-like hydrogel architecture in living cells. Designer DNA nanoparticles are constructed from double-stranded DNA with cytosine-rich stick ends (C-monomer) and are internalized into cells through lysosomal pathway. The lysosomal acidic microenvironment can activate the assembly of DNA monomers, inducing transformation from nanoparticles to micro-sized organelle-like hydrogel which could further escape into cytoplasm. We show how the hydrogel regulates cellular behaviors: cytoskeleton is deformed, cell tentacles are significantly shortened, and cell migration is promoted.
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