Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 16, Pages 11044-11052Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c00152
Keywords
-
Categories
Funding
- NNSF of China [21675130, 21775124, 21675129]
- Chongqing Talents.Touth Top-Notch Personnel Support Program [CQYC201905067]
- Natural Science Foundation Project of CQ CSTC [cstc2018jcyjAX0546]
- Fundamental Research Funds for the Central Universities, China [XDJK2019TJ002]
Ask authors/readers for more resources
Herein, an amphiphilic perylene derivative (denoted as PTC-DEDA) was explored as DNA intercalators endowed with an enhanced affinity and intense electrochemiluminescence (ECL) to construct a target-induced DNA hydrogel biosensing platform for the sensitive detection of microRNA let-7a (miRNA let-7a). Specifically, the DNA hydrogel with numerous dendritic DNA structures was in situ generated via a target-induced nonlinear hybrid chain reaction in the presence of miRNA let-7a, which possessed a large loading capacity to entrap massive DNA intercalators. Then, the PTC-DEDA with positive charges could easily intercalate into the DNA grooves due to the inherent amphipathic structure, achieving a strong ECL signal. Using the proposed PTC-DEDA as both DNA intercalators and ECL emitters, the DNA hydrogel biosensing platform exhibited a high stability and an excellent sensitivity for miRNA let-7a, with a desirable linear range (10 fM to 10 nM) and a low detection limit (1.49 fM). Significantly, the work provides a potential alternative to develop simple and high-efficiency ECL platforms for biochemical analysis applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available