Journal
ANALYTICAL CHEMISTRY
Volume 92, Issue 14, Pages 10033-10041Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c01815
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21775016, 21874013]
- Research Funds for the Central Universities [N170502003, N170908001, N2005027, N182410008-1]
- Talent Project of Revitalizing Liaoning [XLYC1807165]
Ask authors/readers for more resources
Nanozymes have been used in colorimetric and electrochemical sensing because of their low cost and high stability. However, the wide applications of nanozymes in sensing devices are largely limited due to their poor selectivity. In this study, unlike traditional methods using prepared nanozymes for target detection, we designed a target-driven nanozyme growth strategy in TiO2 nanochannels to detect analytes. Using telomerase as an example, the established recognition event was used to expand the photocatalytic activity of TiO2 to visible-light region, thus triggering Prussian blue nanoparticle (PBNP) growth in visible light. Benefiting from the peroxidase (POD)-like activity of PBNPs, the uncharged 3,5,3',5'-tetramethylbenzidine (TMB) is oxidized to positively charged oxTMB, which induces significant ionic transport changes in nanochannels, and thus in turn provides information about telomerase activity. Such a nanozyme-triggered sensing system exhibited excellent performance in telomerase detection in urine specimens from patients with bladder cancer. This innovative target-driven signal generation strategy might provide a new method for applying nanozymes in developing sensitive, rapid, and accurate biological sensing systems.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available