Journal
ANALYTICAL BIOCHEMISTRY
Volume 608, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2020.113875
Keywords
Camel; mWasabi fluorescent protein; Nanobody; VHH
Funding
- Chinese National Natural Science Foundation of China [31070706]
- Fund from the Applied Basic Research Programs of Science and Technology Commission Foundation of Jiangsu Province [BK20161416]
Ask authors/readers for more resources
mWasabi is a bright monomeric green fluorescent protein. It can be used as a fusion tag to monitor various biological events, e.g. protein localization. Here we report the selection of camelid-derived single-domain antibody fragments (nanobodies) against mWasabi. In this work, phage-display approach was employed to select the high affinity mWasabi-specific Nb (nanobodies). These nanobodies were able to recognize mWasabi or in a fused fashion with PD1. The interesting binding characteristics of these two mWasabi-specific nanobodies could be valuable for design new tools for cellular tracing or targeting based on the mWasabi-fusing protein in many different biological research fields.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available