Journal
ANALYTICA CHIMICA ACTA
Volume 1126, Issue -, Pages 1-6Publisher
ELSEVIER
DOI: 10.1016/j.aca.2020.06.007
Keywords
Loop-mediated isothermal amplification; miRNA detection; Phosphorothioated modifications; Self-priming; Terminal hairpin
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Funding
- National Key Project of China [2017YFC0908600]
- National Natural Science Foundation of China [81773817]
- Fundamental Research Funds for the Central Universities [2019FZA7017]
- Leading Talent of Ten Thousand Plan-National High-Level Talents Special Support Plan
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A novel reverse transcription-based loop-mediated isothermal amplification (LAMP) strategy for miRNA detection has been developed. This method consists of two stem-loop probes inspired by the dumbbell-shaped amplicons and inner primers used in conventional LAMP reactions. Termed terminal hairpin formation and self-priming (THSP), this reaction incorporates phosphorothioated (PS) modifications to achieve DNA folding and extension without primers. The final signal is monitored by a sequence-specific detection probe, which minimizes the background noise. We suggest that our rapid, facile, and reliable LAMP method will be a promising candidate for detecting miRNA in biomedical applications. (C) 2020 Elsevier B.V. All rights reserved.
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