Journal
ANALYTICA CHIMICA ACTA
Volume 1127, Issue -, Pages 39-48Publisher
ELSEVIER
DOI: 10.1016/j.aca.2020.06.039
Keywords
Glutathione; Cysteine; Homocysteine; Biomodal ratiometric probe; Redox reaction
Categories
Funding
- Fundamental Research Funds for the Northwest AF University [Z111021601]
- Talented Program [A279021724]
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Herein, a novel bimodal ratiometric probe for sensitive and selective detection of biothiols (including glutathione (GSH), cysteine (Cys) and homocysteine (Hcys)) was constructed, which was based on the redox reaction between manganese dioxide nanoflakes (MnO2 NFs) and rhodamine (RhB) and biothiols. When MnO2 NFs was added into RhB solution, RhB was oxidized to a series of derivatives, accompanying with the colorimetric color changing from purple to light pink and fluorescence changing from red to green. In the presence of GSH, Cys or Hcys, they could reduce MnO2 NFs to Mn2+, thereby preventing the following oxidization of RhB and the corresponding color and fluorescence changes. The absorption intensity ratio and fluorescence intensity ratio showed good linear relationships with the concentrations of biothiols. The colorimetric detection limits for GSH, Cys and Hcys were 0.057 mu M, 0.140 mu M and 0.087 mM, respectively. And the fluorescence detection limits were 0.177 nu M, 0.282 mu M and 0.161 mu M. More importantly, this probe was successfully applied to monitor the concentration of GSH/Cys/Hcys in human serum samples, with satisfactory recovery. Thus, this MnO2 NFs-RhB platform can potentially be a candidate for the detection of biothiols. (C) 2020 Elsevier B.V. All rights reserved.
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