4.5 Article

Positive Effects of Qing'e Pill on Trabecular Microarchitecture and its Mechanical Properties in Osteopenic Ovariectomised Mice

Journal

CHINESE JOURNAL OF INTEGRATIVE MEDICINE
Volume 25, Issue 4, Pages 270-277

Publisher

SPRINGER
DOI: 10.1007/s11655-016-2604-0

Keywords

ovariectimised; osteoporosis; micro-Computed tomography; oestradiol; Qing'e Pill; Chinese medicine; -catenin

Funding

  1. Innovation Fund of Huazhong University of Science and Technology [2013QN235]
  2. National Natural Science Foundation of China [81403257, 81273907, 81102692, 81072493]

Ask authors/readers for more resources

ObjectiveTo investigate the impact of Qing'e Pill (QEP) on the cancellous bone microstructure and its effect on the level of -catenin in a mouse model of postmenopausal osteoporosis.MethodsNinety-six 8-week-old specific pathogen free C57BL/6 mice were randomly divided into 4 groups (24/group): sham, ovariectomised osteoporosis model, oestradiol-treated, and QEP-treated groups. Three months after surgery, the third lumbar vertebra and left femur of the animals were dissected and scanned using micro-computed tomography (micro-CT) to acquire three-dimensional (3D) parameters of their cancellous bone microstructure. The impact of ovariectomy, the effect of oestradiol and QEP intervention on cancellous bone microstructure, and the expression of -catenin were evaluated.ResultsThe oestradioland the QEP-treated groups exhibited a significant increase in the bone volume fraction, trabecular number, trabecular thickne ss s, bone surface to bone volume ratio (BS/BV), and -catenin expression compared with those of the model group (P <0.05). In contrast, the structure model index, trabecular separation, and BS/BV were significantly decreased compared with those of the ovariectomised osteoporosis model group (P <0.05). No differences were observed in the above parameters between animals of the QEP- and oestradiol-treated groups.ConclusionsThe increased -catenin expression may be the mechanism underlying QEP's improvement of the cancellous bone microstructure in ovariectomised mice. Our findings provide a scientific rationale for using QEP as a dietary supplement to prevent bone loss in postmenopausal women.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available