Journal
AICHE JOURNAL
Volume 66, Issue 12, Pages -Publisher
WILEY
DOI: 10.1002/aic.16540
Keywords
anaerobic fungi; Arduino; automatic culture equipment; non-model microbe; pressure transducer
Categories
Funding
- DOE [DE-AC02-05CH11231]
- Engineering and Physical Sciences Research Council [EP/S001581/1]
- National Science Foundation [MCB-1553721]
- EPSRC [EP/S001581/2, EP/S001581/1] Funding Source: UKRI
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Measuring the growth rate of non-model anaerobic microbes typically requires the use of time-consuming and often destructive manual measurements. Here, an Arduino based automatic pressure evaluation system (A-APES) was developed to automatically measure the rate of fermentation gas production as a proxy for microbial growth in anaerobic systems. The A-APES system measures accumulated gas pressure in sealed cultures accurately at high-resolution, while venting the system at programmed intervals to prevent over pressurization. The utility of A-APES is demonstrated in this study by quantifying the growth rate and phases of a biomass-degrading anaerobic gut fungus, which cannot be otherwise measured via conventional techniques due to its association with particulate substrates. Given the utility of the A-APES approach, we provide a complete construction guide to fabricate the device, which is three times less expensive compared to existing commercial alternatives.
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