Journal
AGING-US
Volume 12, Issue 11, Pages 10736-10753Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/aging.103290
Keywords
steroid deficiency-induced osteoporosis; gut microbiota; 16S rRNA sequencing; metagenomics; ovariectomized rats
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Funding
- Interdisciplinary Research of 9th People's Hospital [JYJC201809]
- Shanghai Clinical Medical Center [2017ZZ01023]
- Shanghai Municipal Key Clinical Specialty
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As a critical factor involved in the maintenance of physiological homeostasis, the gut microbiota (GM) reportedly plays a key role in bone development. To date, the association between the GM and steroid deficiency-induced osteoporosis remains poorly understood. Forty female Sprague Dawley rats were divided into an ovariectomy (OVX) or control group. We performed 16S rRNA and metagenome sequencing, to compare diversity, taxonomic differences, and functional genes. The GM composition did not change in the control group and the number of operational taxonomic units increased significantly following ovariectomy. Alpha diversity, determined by ACE estimator, CHAO estimator, the Shannon index, and the Simpson index showed an increasing trend after ovariectomy. Samples in the OVX group were well clustered both pre- and post-ovariectomy, as demonstrated by principal coordinate 1 (PC1) and PC2. Functional genes of GM, including those involved in synthesis and metabolism of carbohydrates and nucleotides, microbial structure, and heme, as well as hemin uptake and utilization, increased at the early stage of osteoporosis. We observed that Ruminococcus flavefaciens exhibited the greatest variation in abundance among the GM and this was also associated with osteoclastic indicators and the estrobolome. Specific changes in fecal microbiota are associated with the pathogenesis of steroid deficiency-induced osteoporosis.
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