4.8 Article

Self-Degradable Lipid-Like Materials Based on Hydrolysis accelerated by the intra-Particle Enrichment of Reactant (HyPER) for Messenger RNA Delivery

Journal

ADVANCED FUNCTIONAL MATERIALS
Volume 30, Issue 34, Pages -

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201910575

Keywords

ionizable lipid; messenger RNA; nanoemulsion; self-degradability

Funding

  1. JSPS KAKENHI [17H06558, 18K18377, 20H00657]
  2. Inohana Foundation (Chiba University)
  3. JST CREST grant [JPMJCR17H1]
  4. Institute for Global Prominent Research (Chiba University)
  5. Takeda Science Foundation
  6. Asahi Glass Foundation
  7. [hp190119]
  8. Grants-in-Aid for Scientific Research [18K18377, 20H00657, 17H06558] Funding Source: KAKEN

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RNA-based therapeutics is a promising approach for curing intractable diseases by manipulating various cellular functions. For eliciting RNA (i.e., mRNA and siRNA) functions successfully, the RNA in the extracellular space must be protected and it must be delivered to the cytoplasm. In this study, the development of a self-degradable lipid-like material that functions to accelerate the collapse of lipid nanoparticles (LNPs) and the release of RNA into cytoplasm is reported. The self-degradability is based on a unique reaction Hydrolysis accelerated by intra-Particle Enrichment of Reactant (HyPER). In this reaction, a disulfide bond and a phenyl ester are essential structural components: concentrated hydrophobic thiols that are produced by the cleavage of the disulfide bonds in the LNPs drive an intraparticle nucleophilic attack to the phenyl ester linker, which results in further degradation. An oleic acid-scaffold lipid-like material that mounts all of these units (ssPalmO-Phe) shows superior transfection efficiency to nondegradable or conventional materials. The insertion of the aromatic ring is unexpectedly revealed to contribute to the enhancement of endosomal escape. Since the intracellular trafficking is a sequential process that includes cellular uptake, endosomal escape, the release of mRNA, and translation, the improvement in each process synergistically enhances the gene expression.

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