Journal
BLOOD
Volume 126, Issue 13, Pages 1601-1608Publisher
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2015-04-641654
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Funding
- Wellcome Trust [073107, 088410]
- British Heart Foundation [PG/07/116, PG/05/134]
- Najran University [DLAB ROEH16283]
- Emmy Noether grant from the Deutsche Forschungsgemeinschaft [BE5084/3-1]
- British Heart Foundation [RG/13/18/30563] Funding Source: researchfish
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The glycoprotein VI (GPVI)-Fc receptor gamma (FcR gamma) chain is the major platelet signaling receptor for collagen. Paradoxically, in a FeCl3 injury model, occlusion, but not initiation of thrombus formation, is delayed in GPVI-deficient and GPVI-depleted mice. In this study, we demonstrate that GPVI is a receptor for fibrin and speculate that this contributes to development of an occlusive thrombus. We observed a marked increase in tyrosine phosphorylation, including the FcR gamma chain and Syk, in human and mouse platelets induced by thrombin in the presence of fibrinogen and the alpha IIb beta 3 blocker eptifibatide. This was not seen in platelets stimulated by a protease activated receptor (PAR)-4 peptide, which is unable to generate fibrin from fibrinogen. The pattern of tyrosine phosphorylation was similar to that induced by activation of GPVI. Consistent with this, thrombin did not induce tyrosine phosphorylation of Syk and the FcR gamma chain in GPVI-deficient mouse platelets. Mouse platelets underwent full spreading on fibrin but not fibrinogen, which was blocked in the presence of a Src kinase inhibitor or in the absence of GPVI. Spreading on fibrin was associated with phosphatidylserine exposure (procoagulant activity), and this too was blocked in GPVI-deficient platelets. The ectodomain of GPVI was shown to bind to immobilized monomeric and polymerized fibrin. A marked increase in embolization was seen following FeCl3 injury in GPVI-deficient mice, likely contributing to the delay in occlusion in this model. These results demonstrate that GPVI is a receptor for fibrin and provide evidence that this interaction contributes to thrombus growth and stability.
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