Journal
ACS CHEMICAL BIOLOGY
Volume 15, Issue 7, Pages 1862-1873Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.0c00100
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Funding
- Ministry of Science and Technology (MOST) [107-2628-M-002-017]
- NATO Science for Peace and Security Programme [SPS 985291]
- European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant [690853, 796245]
- NSFC/China [21905090]
- Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning
- Natural Science Foundation of Shanghai [19ZR1412200]
- Marie Curie Actions (MSCA) [796245] Funding Source: Marie Curie Actions (MSCA)
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We demonstrate the construction of wavelength lambda-ratiometric images that allow visualizing the distribution of microscopic dynamics within living cells and tissues by using the newly developed principle of fluorescence response. The bent-to-planar motion in the excited state of incorporated fluorescence probes leads to elongation of the pi-delocalization, resultin in microviscosity-dependent but polarity-insensitive interplay between well-separated blue and red bands in emission spectra. This allows constructing the exceptionally contrasted images of cellular dynamics. Moreover, the application of probes with increased affinity toward biological membranes allowed detecting the differences in dynamics between the plasma membrane and intracellular membrane structures. Such lambda-ratiometric microviscosity imaging was extended for mapping the living tissues and observing their inflammation-dependent changes.
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