4.6 Article

Effect of the His-Tag Location on Decapping Scavenger Enzymes and Their Hydrolytic Activity toward Cap Analogs

Journal

ACS OMEGA
Volume 5, Issue 19, Pages 10759-10766

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acsomega.0c00304

Keywords

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Funding

  1. Polish National Science Centre [UMO/2013/08/A/NZ1/00866, UMO/2017/27/B/NZ1/01859]
  2. National Centre of Research and Development [STRATEGMED 1/235773/19/NCBR/2016]

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Decapping scavenger enzymes (DcpSs) are important players in mRNA degradation machinery and conserved in eukaryotes. Importantly, human DcpS is the recognized target for spinal muscular atrophy (SMA) and acute myeloid leukemia (AML) therapy, and has recently been connected to development of intellectual disability. Most recombinant DcpSs used in biochemical and biophysical studies are prepared as tagged proteins, with polyhistidine (His-tag) at the N-terminus or C-terminus. Our work is the first report on the parallel characterization of three versions of DcpSs (native and N- or C-terminally tagged) of three species (humans, Caenorhabditis elegans, and Ascaris suum). The native forms of all three enzymes were prepared by N-(His)(10) tag cleavage. Protein thermal stability, measured by differential scanning fluorimetry (DSF), was unaffected in the case of native and tagged versions of human and A. suum DcpS; however, the melting temperature (T-m) of C. elagans DcpS of was significantly influenced by the presence of the additional N- or C-tag. To investigate the impact of the tag positioning on the catalytic properties of DcpS, we tested the hydrolytic activity of native DcpS and their His-tagged counterparts toward cap dinucleotides (m(7)GpppG and m(3) (2,2,7)GpppG) and m(7)GDP. The kinetic data indicate that dinucleotide substrates are hydrolyzed with comparable efficiency by native human and A. suum DcpS and their His-tagged forms. In contrast, both His-tagged C. elegans DcpSs exhibited higher activity toward m(7)GpppG than the native enzyme. m(7)GDP is resistant to enzymatic cleavage by all three forms of human and nematode DcpS.

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