Journal
BIOMOLECULES
Volume 10, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/biom10050763
Keywords
membrane protein; electron paramagnetic resonance (EPR); site-directed spin labeling; structural and dynamics; membrane mimetic; double electron electron resonance (DEER)
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Funding
- NIGMS/NIH Maximizing Investigator's Research Award (MIRA) [R35 GM126935]
- NSF [CHE-1807131]
- John W. Steube Professorship
- Campbellsville University
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Membrane proteins possess a variety of functions essential to the survival of organisms. However, due to their inherent hydrophobic nature, it is extremely difficult to probe the structure and dynamic properties of membrane proteins using traditional biophysical techniques, particularly in their native environments. Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a very powerful and rapidly growing biophysical technique to study pertinent structural and dynamic properties of membrane proteins with no size restrictions. In this review, we will briefly discuss the most commonly used EPR techniques and their recent applications for answering structure and conformational dynamics related questions of important membrane protein systems.
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