Journal
MICROBIAL GENOMICS
Volume 6, Issue 4, Pages -Publisher
MICROBIOLOGY SOC
DOI: 10.1099/mgen.0.000352
Keywords
Escherichia coli; ST131; genomics; antimicrobial resistance; long-term care facilities
Categories
Funding
- Dublin City University (DCU) O'Hare PhD fellowship
- DCU Enhancing Performance grant
- Health Innovation Challenge Fund [WT098600, HICF-T5-342]
- Department of Health
- Wellcome Trust
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Escherichia coli sequence type 131 (ST131) is a pandemic clone that is evolving rapidly with increasing levels of antimicrobial resistance. Here, we investigated an outbreak of E. coli ST131 producing extended spectrum beta-lactamases (ESBLs) in a long-term care facility (LTCF) in Ireland by combining data from this LTCF (n=69) with other Irish (n=35) and global (n=690) ST131 genomes to reconstruct the evolutionary history and understand changes in population structure and genome architecture over time. This required a combination of short- and long-read genome sequencing, de novo assembly, read mapping, ESBL gene screening, plasmid alignment and temporal phylogenetics. We found that Clade C was the most prevalent (686 out of 794 isolates, 86%) of the three major ST131 clades circulating worldwide (A with fimH41, B with fimH22, C with fimH30), and was associated with the presence of different ESBL alleles, diverse plasmids and transposable elements. Clade C was estimated to have emerged in c. 1985and subsequently acquired different ESBL gene variants (bla(CTX-M-14) vs bla(CTX-M-15)). An ISEcpl-mediated transposition of the bla(CTX-M-15) gene further increased the diversity within Clade C. We discovered a local clonal expansion of a rare C2 lineage (C2_8) with a chromosomal insertion of blaCTX-M-15 at the mppA gene. This was acquired from an lncFIA plasmid. The C2_8 lineage clonally expanded in the Irish LTCF from 2006. displacing the existing C1 strain (C1_10). highlighting the potential for novel ESBL-producing ST131 with a distinct genetic profile to cause outbreaks strongly associated with specific healthcare environments.
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