Journal
SLAS TECHNOLOGY
Volume 25, Issue 5, Pages 436-445Publisher
ELSEVIER SCIENCE INC
DOI: 10.1177/2472630320915837
Keywords
3D cell culture; spheroids; alginate; centrifugal droplet generation; cell encapsulation
Funding
- University of New Mexico
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-SC0001035]
- National Cancer Institute of the National Institutes of Health [5 F31 CA189682]
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Quickly and easily producing uniform populations of microsphere-based 3D cell cultures using droplet-based templating methods has the potential to enable widespread use of such platforms in drug discovery or cancer research. Here, we advance the design of centrifuge-based droplet generation devices, describe the use of this platform for droplet generation with controlled cell occupancy, and demonstrate weeklong culture duration. Using simple-to-construct devices and easily implemented protocols, the initial concentration of encapsulated cells is adjustable up to hundreds of cells per microsphere. This work demonstrates the first instance of using centrifugal droplet-generating devices to produce large numbers of cell-encapsulating microspheres. Applications of this versatile methodology include the rapid formation of templated 3D cell culture populations suitable for suspension culture or large batch bioreactor studies that require uniform populations.
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